A secure platform for developing and sharing reproducible methods.https://protocols.io/protocols.rssprotocols.ioen© 2026 protocols.io, part of Springer Nature. All rights reservedprotocols.io© 2026 protocols.io, part of Springer Nature. All rights reserved.info@protocols.iowww.protocols.io/view/targeted-capture-long-read-amplicon-sequencing-b7yhrpt6protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.dm6gpbybdlzp/v1

Targeted capture long-read amplicon sequencing ]]>Targeted capture long-read amplicon sequencing Myo Naungdx.doi.org/10.17504/protocols.io.dm6gpbybdlzp/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.dm6gpbybdlzp/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.dm6gpbybdlzp/v1www.protocols.io/view/targeted-capture-long-read-amplicon-sequencing-b7yhrpt6www.protocols.io/view/multiplexed-immunofluorescence-phenocycler-fusion-gz5xbx87pprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.q26g79ep1vwz/v1

Multiplexed Immunofluorescence Phenocycler-Fusion® (CODEX) Imaging of FFPE Female Reproductive Organs]]>Multiplexed Immunofluorescence Phenocycler-Fusion® (CODEX) Imaging of FFPE Female Reproductive OrgansDiane Saundersdx.doi.org/10.17504/protocols.io.q26g79ep1vwz/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.q26g79ep1vwz/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.q26g79ep1vwz/v1www.protocols.io/view/multiplexed-immunofluorescence-phenocycler-fusion-gz5xbx87pwww.protocols.io/view/yale-murine-tmc-mouse-thymus-and-spleen-sample-sel-hdfyb23pxprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.5qpvoddoxg4o/v1

Yale Murine TMC - Mouse thymus and spleen sample selection and preparation protocol ]]>Yale Murine TMC - Mouse thymus and spleen sample selection and preparation protocol Jungmin Namdx.doi.org/10.17504/protocols.io.5qpvoddoxg4o/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.5qpvoddoxg4o/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.5qpvoddoxg4o/v1www.protocols.io/view/yale-murine-tmc-mouse-thymus-and-spleen-sample-sel-hdfyb23pxwww.protocols.io/view/an-isolation-culture-and-stimulation-method-of-suc-hmfrb43m7protocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.j8nlk1o25g5r/v1

An isolation, culture and stimulation method of suckling rabbit cecal immune enriched lamina propria cells]]>An isolation, culture and stimulation method of suckling rabbit cecal immune enriched lamina propria cellsChristelle Knudsendx.doi.org/10.17504/protocols.io.j8nlk1o25g5r/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.j8nlk1o25g5r/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.j8nlk1o25g5r/v1www.protocols.io/view/an-isolation-culture-and-stimulation-method-of-suc-hmfrb43m7www.protocols.io/view/agarose-embedding-of-samples-for-lavision-umii-hr3gb58jxprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.5jyl8xkzrv2w/v1

Agarose embedding of samples for LaVision UMII]]>Agarose embedding of samples for LaVision UMIIDeniz Bekatdx.doi.org/10.17504/protocols.io.5jyl8xkzrv2w/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.5jyl8xkzrv2w/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.5jyl8xkzrv2w/v1www.protocols.io/view/agarose-embedding-of-samples-for-lavision-umii-hr3gb58jxwww.protocols.io/view/divas-dna-isolation-for-various-species-96-wells-f-jzpdcp5i7protocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.ewov1rr8plr2/v1

DIVAS : DNA Isolation for VArious Species (96 wells format)]]>DIVAS : DNA Isolation for VArious Species (96 wells format) Christophe Bourydx.doi.org/10.17504/protocols.io.ewov1rr8plr2/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.ewov1rr8plr2/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.ewov1rr8plr2/v1www.protocols.io/view/divas-dna-isolation-for-various-species-96-wells-f-jzpdcp5i7www.protocols.io/view/18s-metabarcoding-for-plankton-in-marine-sediment-j3cpcqivpprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.14egnpxxpv5d/v1

18S Metabarcoding for Plankton in Marine Sediment Samples ]]>18S Metabarcoding for Plankton in Marine Sediment Samples Max MT Titcombdx.doi.org/10.17504/protocols.io.14egnpxxpv5d/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.14egnpxxpv5d/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.14egnpxxpv5d/v1www.protocols.io/view/18s-metabarcoding-for-plankton-in-marine-sediment-j3cpcqivpwww.protocols.io/view/restriction-enzyme-based-dna-concatenation-protoco-j4qecqvtfprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.rm7vzw3oxvx1/v1

Restriction Enzyme Based DNA Concatenation Protocol for Short Synthetic DNA Fragments]]>Restriction Enzyme Based DNA Concatenation Protocol for Short Synthetic DNA FragmentsKornelija Kaminskaitėdx.doi.org/10.17504/protocols.io.rm7vzw3oxvx1/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.rm7vzw3oxvx1/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.rm7vzw3oxvx1/v1www.protocols.io/view/restriction-enzyme-based-dna-concatenation-protoco-j4qecqvtfwww.protocols.io/view/effect-of-bioceramic-and-calcium-hydroxide-intraca-j69wcrh7fprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.6qpvr19xbgmk/v1

Effect of Bioceramic and Calcium Hydroxide Intracanal Medications on Dentinal Tubule Penetration and Push-Out Bond Strength of a Bioceramic Root Canal Sealer: An In-Vitro Study]]>Effect of Bioceramic and Calcium Hydroxide Intracanal Medications on Dentinal Tubule Penetration and Push-Out Bond Strength of a Bioceramic Root Canal Sealer: An In-Vitro StudyOmar Yasser Elsayed Elarabydx.doi.org/10.17504/protocols.io.6qpvr19xbgmk/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.6qpvr19xbgmk/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.6qpvr19xbgmk/v1www.protocols.io/view/effect-of-bioceramic-and-calcium-hydroxide-intraca-j69wcrh7fwww.protocols.io/view/high-throughput-total-phenolic-content-assay-of-pl-j762crrgfprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.e6nvwxeq7gmk/v1

High-throughput Total Phenolic Content Assay of Plant Tissue]]>High-throughput Total Phenolic Content Assay of Plant TissueHenry A. A Fryedx.doi.org/10.17504/protocols.io.e6nvwxeq7gmk/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.e6nvwxeq7gmk/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.e6nvwxeq7gmk/v1www.protocols.io/view/high-throughput-total-phenolic-content-assay-of-pl-j762crrgfwww.protocols.io/view/emergency-care-avoidance-and-delays-in-the-united-j83ccryixprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.kqdg3r397g25/v1

Emergency Care Avoidance and Delays in the United States: Scoping Review Protocol]]>Emergency Care Avoidance and Delays in the United States: Scoping Review ProtocolElaine Hsiangdx.doi.org/10.17504/protocols.io.kqdg3r397g25/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.kqdg3r397g25/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.kqdg3r397g25/v1www.protocols.io/view/emergency-care-avoidance-and-delays-in-the-united-j83ccryixwww.protocols.io/view/automated-opentrons-flex-pierce-dilution-free-rapi-j9n4cr5gxprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.5jyl83627v2w/v1

Automated (Opentrons Flex) Pierce™ Dilution-Free™ Rapid Gold BCA Protein Assay Kit]]>Automated (Opentrons Flex) Pierce™ Dilution-Free™ Rapid Gold BCA Protein Assay KitDAMP Labdx.doi.org/10.17504/protocols.io.5jyl83627v2w/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.5jyl83627v2w/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.5jyl83627v2w/v1www.protocols.io/view/automated-opentrons-flex-pierce-dilution-free-rapi-j9n4cr5gxwww.protocols.io/view/10-ont-library-qc-and-pooling-for-metagenomics-seq-j9pncr5mfprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.x54v99krzv3e/v1

10. ONT library QC and pooling for metagenomics sequencing (mNGS)]]>10. ONT library QC and pooling for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.x54v99krzv3e/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.x54v99krzv3e/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.x54v99krzv3e/v1www.protocols.io/view/10-ont-library-qc-and-pooling-for-metagenomics-seq-j9pncr5mfwww.protocols.io/view/08-ont-rna-library-preparation-for-metagenomics-se-j9ppcr5mpprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.eq2lym96qlx9/v1

08. ONT RNA library preparation for metagenomics sequencing (mNGS)]]>08. ONT RNA library preparation for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.eq2lym96qlx9/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.eq2lym96qlx9/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.eq2lym96qlx9/v1www.protocols.io/view/08-ont-rna-library-preparation-for-metagenomics-se-j9ppcr5mpwww.protocols.io/view/09-ont-dna-library-preparation-for-metagenomics-se-j9pqcr5mxprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.bp2l6ordzlqe/v1

09. ONT DNA library preparation for metagenomics sequencing (mNGS)]]>09. ONT DNA library preparation for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.bp2l6ordzlqe/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.bp2l6ordzlqe/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.bp2l6ordzlqe/v1www.protocols.io/view/09-ont-dna-library-preparation-for-metagenomics-se-j9pqcr5mxwww.protocols.io/view/appendix-a-secondary-library-amplification-for-ill-j9prcr5m7protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.j8nlk7n91g5r/v1

Appendix A. Secondary library amplification for Illumina]]>Appendix A. Secondary library amplification for IlluminaGowtham Thakkudx.doi.org/10.17504/protocols.io.j8nlk7n91g5r/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.j8nlk7n91g5r/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.j8nlk7n91g5r/v1www.protocols.io/view/appendix-a-secondary-library-amplification-for-ill-j9prcr5m7www.protocols.io/view/07-iseq-100-loading-for-metagenomics-sequencing-mn-j9ptcr5npprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.14egnpe9qv5d/v1

07. iSeq 100 loading for metagenomics sequencing (mNGS)]]>07. iSeq 100 loading for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.14egnpe9qv5d/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.14egnpe9qv5d/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.14egnpe9qv5d/v1www.protocols.io/view/07-iseq-100-loading-for-metagenomics-sequencing-mn-j9ptcr5npwww.protocols.io/view/06-illumina-library-qc-and-pooling-for-metagenomic-j9pucr5nxprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.5jyl836d7v2w/v1

06. Illumina library QC and pooling for metagenomics sequencing (mNGS)]]>06. Illumina library QC and pooling for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.5jyl836d7v2w/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.5jyl836d7v2w/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.5jyl836d7v2w/v1www.protocols.io/view/06-illumina-library-qc-and-pooling-for-metagenomic-j9pucr5nxwww.protocols.io/view/05-illumina-dna-library-preparation-for-metagenomi-j9pvcr5n7protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.8epv5wb24v1b/v1

05. Illumina DNA library preparation for metagenomics sequencing (mNGS) - NEB kit]]>05. Illumina DNA library preparation for metagenomics sequencing (mNGS) - NEB kitGowtham Thakkudx.doi.org/10.17504/protocols.io.8epv5wb24v1b/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.8epv5wb24v1b/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.8epv5wb24v1b/v1www.protocols.io/view/05-illumina-dna-library-preparation-for-metagenomi-j9pvcr5n7www.protocols.io/view/04-neb-illumina-rna-library-preparation-for-metage-j9pxcr5ppprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.q26g7qdmqlwz/v1

04-NEB. Illumina RNA library preparation for metagenomics sequencing (mNGS) - NEB kit]]>04-NEB. Illumina RNA library preparation for metagenomics sequencing (mNGS) - NEB kitGowtham Thakkudx.doi.org/10.17504/protocols.io.q26g7qdmqlwz/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.q26g7qdmqlwz/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.q26g7qdmqlwz/v1www.protocols.io/view/04-neb-illumina-rna-library-preparation-for-metage-j9pxcr5ppwww.protocols.io/view/03-nucleic-acid-quality-control-for-metagenomics-s-j9pycr5pxprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.kxygxro7kg8j/v1

03. Nucleic acid quality control for metagenomics sequencing (mNGS)]]>03. Nucleic acid quality control for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.kxygxro7kg8j/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.kxygxro7kg8j/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.kxygxro7kg8j/v1www.protocols.io/view/03-nucleic-acid-quality-control-for-metagenomics-s-j9pycr5pxwww.protocols.io/view/02-nucleic-acid-extraction-and-dnase-treatment-for-j9pzcr5p7protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.81wgbm2n1vpk/v1

02. Nucleic acid extraction and DNase treatment for metagenomics sequencing (mNGS)]]>02. Nucleic acid extraction and DNase treatment for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.81wgbm2n1vpk/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.81wgbm2n1vpk/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.81wgbm2n1vpk/v1www.protocols.io/view/02-nucleic-acid-extraction-and-dnase-treatment-for-j9pzcr5p7www.protocols.io/view/01-sample-collection-storage-and-preparation-for-m-j9p2cr5qfprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.6qpvr1rkogmk/v1

01. Sample collection, storage and preparation for metagenomics sequencing (mNGS)]]>01. Sample collection, storage and preparation for metagenomics sequencing (mNGS)Gowtham Thakkudx.doi.org/10.17504/protocols.io.6qpvr1rkogmk/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.6qpvr1rkogmk/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.6qpvr1rkogmk/v1www.protocols.io/view/01-sample-collection-storage-and-preparation-for-m-j9p2cr5qfwww.protocols.io/view/11-ont-adapter-ligation-and-flow-cell-priming-and-j9qwcr5xfprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.6qpvr1rp3gmk/v1

11. ONT adapter ligation and flow cell priming and loading]]>11. ONT adapter ligation and flow cell priming and loadingGowtham Thakkudx.doi.org/10.17504/protocols.io.6qpvr1rp3gmk/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.6qpvr1rp3gmk/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.6qpvr1rp3gmk/v1www.protocols.io/view/11-ont-adapter-ligation-and-flow-cell-priming-and-j9qwcr5xfwww.protocols.io/view/appendix-e-ont-diagnostic-pcr-for-library-size-ass-j9qxcr5xpprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.n92ldo2w8g5b/v1

Appendix E. ONT diagnostic PCR for library size assessment]]>Appendix E. ONT diagnostic PCR for library size assessmentGowtham Thakkudx.doi.org/10.17504/protocols.io.n92ldo2w8g5b/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.n92ldo2w8g5b/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.n92ldo2w8g5b/v1www.protocols.io/view/appendix-e-ont-diagnostic-pcr-for-library-size-ass-j9qxcr5xpwww.protocols.io/view/appendix-d-spri-bead-quality-control-j9ricr54fprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.261geq2xog47/v1

Appendix D. SPRI bead quality control]]>Appendix D. SPRI bead quality controlGowtham Thakkudx.doi.org/10.17504/protocols.io.261geq2xog47/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.261geq2xog47/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.261geq2xog47/v1www.protocols.io/view/appendix-d-spri-bead-quality-control-j9ricr54fwww.protocols.io/view/detecci-n-molecular-de-la-variante-n314d-c-940a-gt-j9w4cr7gxprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.kxygxroxog8j/v1

Detección molecular de la variante N314D (c.940A>G) en el gen GALT mediante PCR en tiempo real con tecnología TaqMan™ y verificación con Luna Universal Probe qPCR Master Mix]]>Detección molecular de la variante N314D (c.940A>G) en el gen GALT mediante PCR en tiempo real con tecnología TaqMan™ y verificación con Luna Universal Probe qPCR Master MixMaría Paula Chinchilla Trigosdx.doi.org/10.17504/protocols.io.kxygxroxog8j/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.kxygxroxog8j/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.kxygxroxog8j/v1www.protocols.io/view/detecci-n-molecular-de-la-variante-n314d-c-940a-gt-j9w4cr7gxwww.protocols.io/view/x-gal-staining-protocol-for-frozen-sections-of-rat-j9y6cr7zfprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.rm7vzwozxvx1/v1

X-gal Staining Protocol for Frozen Sections of Rat Knee and Goat TMJ Disc attachments]]>X-gal Staining Protocol for Frozen Sections of Rat Knee and Goat TMJ Disc attachmentsMairobys Socorrodx.doi.org/10.17504/protocols.io.rm7vzwozxvx1/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.rm7vzwozxvx1/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.rm7vzwozxvx1/v1www.protocols.io/view/x-gal-staining-protocol-for-frozen-sections-of-rat-j9y6cr7zfwww.protocols.io/view/12-ont-post-sequencing-processing-and-visualizatio-j9zecr73fprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.6qpvr1rj2gmk/v1

12. ONT post-sequencing processing and visualization]]>12. ONT post-sequencing processing and visualizationGowtham Thakkudx.doi.org/10.17504/protocols.io.6qpvr1rj2gmk/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.6qpvr1rj2gmk/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.6qpvr1rj2gmk/v1www.protocols.io/view/12-ont-post-sequencing-processing-and-visualizatio-j9zecr73fwww.protocols.io/view/systematic-review-protocol-for-the-article-acceler-kcbpcssmpprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.n92ldo1bng5b/v1

Systematic review protocol for the article “Accelerated aging protocols for performance assessment of adhered ceramic tile and rendered systems: a systematic literature review”]]>Systematic review protocol for the article “Accelerated aging protocols for performance assessment of adhered ceramic tile and rendered systems: a systematic literature review”Antonio Rinaldo de Oliveira Júniordx.doi.org/10.17504/protocols.io.n92ldo1bng5b/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.n92ldo1bng5b/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.n92ldo1bng5b/v1www.protocols.io/view/systematic-review-protocol-for-the-article-acceler-kcbpcssmpwww.protocols.io/view/00-overview-of-metagenomics-protocols-kh73ct9qpprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.2bvjroxpwlk5/v1

00. Overview of Metagenomics Protocols]]>00. Overview of Metagenomics ProtocolsGowtham Thakkudx.doi.org/10.17504/protocols.io.2bvjroxpwlk5/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.2bvjroxpwlk5/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.2bvjroxpwlk5/v1www.protocols.io/view/00-overview-of-metagenomics-protocols-kh73ct9qpwww.protocols.io/view/04-wm-illumina-rna-library-preparation-for-metagen-kh79ct9r7protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.wov1yj45yvr2/v1

04-WM. Illumina RNA library preparation for metagenomics sequencing (mNGS) - Watchmaker kit]]>04-WM. Illumina RNA library preparation for metagenomics sequencing (mNGS) - Watchmaker kitGowtham Thakkudx.doi.org/10.17504/protocols.io.wov1yj45yvr2/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.wov1yj45yvr2/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.wov1yj45yvr2/v1www.protocols.io/view/04-wm-illumina-rna-library-preparation-for-metagen-kh79ct9r7www.protocols.io/view/rapid-response-metagenomics-protocols-kh8dct9s7protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.8nlk5b4nwl5r/v1

Rapid Response Metagenomics Protocols]]>Rapid Response Metagenomics ProtocolsGowtham Thakkudx.doi.org/10.17504/protocols.io.8nlk5b4nwl5r/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.8nlk5b4nwl5r/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.8nlk5b4nwl5r/v1www.protocols.io/view/rapid-response-metagenomics-protocols-kh8dct9s7www.protocols.io/view/bacterial-transformation-kn6ncvhdfprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.6nvw6po1dgmk/v1

Bacterial Transformation]]>Bacterial TransformationDaniel Ciulladx.doi.org/10.17504/protocols.io.6nvw6po1dgmk/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.6nvw6po1dgmk/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.6nvw6po1dgmk/v1www.protocols.io/view/bacterial-transformation-kn6ncvhdfwww.protocols.io/view/04-ilmn-illumina-rapid-rna-library-prep-illumina-k-kn6zcvhf7protocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.8nlk5bn81l5r/v1

04-ILMN. Illumina Rapid RNA Library Prep - Illumina Kit]]>04-ILMN. Illumina Rapid RNA Library Prep - Illumina KitGenay Pilarowskidx.doi.org/10.17504/protocols.io.8nlk5bn81l5r/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.8nlk5bn81l5r/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.8nlk5bn81l5r/v1www.protocols.io/view/04-ilmn-illumina-rapid-rna-library-prep-illumina-k-kn6zcvhf7www.protocols.io/view/appendix-b-truseq-compatible-mini-adapters-kn62cvhgfprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.m7vznmojrvx1/v1

Appendix B. TruSeq-compatible mini adapters]]>Appendix B. TruSeq-compatible mini adaptersGenay Pilarowskidx.doi.org/10.17504/protocols.io.m7vznmojrvx1/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.m7vznmojrvx1/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.m7vznmojrvx1/v1www.protocols.io/view/appendix-b-truseq-compatible-mini-adapters-kn62cvhgfwww.protocols.io/view/viral-target-capture-sequencing-of-air-samples-kpiwcvkffprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.61ge6p2qjl47/v1

Viral Target Capture Sequencing of Air Samples]]>Viral Target Capture Sequencing of Air SamplesIsla Emmendx.doi.org/10.17504/protocols.io.61ge6p2qjl47/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.61ge6p2qjl47/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.61ge6p2qjl47/v1www.protocols.io/view/viral-target-capture-sequencing-of-air-samples-kpiwcvkffwww.protocols.io/view/an-optimized-protocol-for-cytogenetics-in-stingles-kpq3cvmypprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.4egnxbn6ql5d/v1

An optimized protocol for cytogenetics in stingless bees (Hymenoptera: Apidae: Meliponini)]]>An optimized protocol for cytogenetics in stingless bees (Hymenoptera: Apidae: Meliponini)Weslei Nascimentodx.doi.org/10.17504/protocols.io.4egnxbn6ql5d/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.4egnxbn6ql5d/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.4egnxbn6ql5d/v1www.protocols.io/view/an-optimized-protocol-for-cytogenetics-in-stingles-kpq3cvmypwww.protocols.io/view/reagent-preparation-for-growth-plate-assay-kp96cvr9fprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.m7vznm2x2vx1/v1

Reagent Preparation for Growth Plate Assay]]>Reagent Preparation for Growth Plate AssayDAMP Labdx.doi.org/10.17504/protocols.io.m7vznm2x2vx1/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.m7vznm2x2vx1/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.m7vznm2x2vx1/v1www.protocols.io/view/reagent-preparation-for-growth-plate-assay-kp96cvr9fwww.protocols.io/view/affordable-cardiac-rehabilitation-an-outreach-inte-kqqicvvufprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.qpvrexqezlmk/v1

Affordable Cardiac Rehabilitation: An Outreach Inter-disciplinary Strategic Study (ACROSS): External Pilot Trial]]>Affordable Cardiac Rehabilitation: An Outreach Inter-disciplinary Strategic Study (ACROSS): External Pilot TrialDavid Innesdx.doi.org/10.17504/protocols.io.qpvrexqezlmk/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.qpvrexqezlmk/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.qpvrexqezlmk/v1www.protocols.io/view/affordable-cardiac-rehabilitation-an-outreach-inte-kqqicvvufwww.protocols.io/view/zymopure-plasmid-miniprep-kit-protocol-kqvgcvw3xprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.qpvonxw99l4o/v1

ZymoPURE Plasmid Miniprep Kit Protocol]]>ZymoPURE Plasmid Miniprep Kit ProtocolDaniel Ciulladx.doi.org/10.17504/protocols.io.qpvonxw99l4o/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.qpvonxw99l4o/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.qpvonxw99l4o/v1www.protocols.io/view/zymopure-plasmid-miniprep-kit-protocol-kqvgcvw3xwww.protocols.io/view/ampicillin-sulbactam-versus-cefiderocol-versus-the-kqwfcvxbpprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.q2lydzqxqlx9/v1

Ampicillin/sulbactam versus cefiderocol versus their combination in the treatment of carbapenem-resistant AcinetoBactEr bAumannii bloodSTream infections: a multicentre observational study with target trial emulation (A-BEAST study)]]>Ampicillin/sulbactam versus cefiderocol versus their combination in the treatment of carbapenem-resistant AcinetoBactEr bAumannii bloodSTream infections: a multicentre observational study with target trial emulation (A-BEAST study)Giuseppe Pipitonedx.doi.org/10.17504/protocols.io.q2lydzqxqlx9/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.q2lydzqxqlx9/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.q2lydzqxqlx9/v1www.protocols.io/view/ampicillin-sulbactam-versus-cefiderocol-versus-the-kqwfcvxbpwww.protocols.io/view/cytotoxicity-and-biodistribution-of-nanoparticles-kqwkcvxcxprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.jyl8nrqe7l2w/v1

Cytotoxicity and biodistribution of nanoparticles in C. elegans (compiled protocols for polystyrene nanoparticles)]]>Cytotoxicity and biodistribution of nanoparticles in C. elegans (compiled protocols for polystyrene nanoparticles)Fabio Ugolinidx.doi.org/10.17504/protocols.io.jyl8nrqe7l2w/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.jyl8nrqe7l2w/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.jyl8nrqe7l2w/v1www.protocols.io/view/cytotoxicity-and-biodistribution-of-nanoparticles-kqwkcvxcxwww.protocols.io/view/takarabio-protocols-smart-seq-total-rna-library-pr-kqyfcvxtpprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.92ld3bn28g5b/v1

TakaraBio Protocols: SMART-Seq® Total RNA Library Prep with ZapR® Depletion (with UMIs) (072126)-- University of Minnesota TMCs]]>TakaraBio Protocols: SMART-Seq® Total RNA Library Prep with ZapR® Depletion (with UMIs) (072126)-- University of Minnesota TMCsMac Andersondx.doi.org/10.17504/protocols.io.92ld3bn28g5b/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.92ld3bn28g5b/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.92ld3bn28g5b/v1www.protocols.io/view/takarabio-protocols-smart-seq-total-rna-library-pr-kqyfcvxtpwww.protocols.io/view/characterization-of-the-physicochemical-properties-kqyhcvxt7protocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.m6gp8eqxdlzp/v1

Characterization of the physicochemical properties of Polystyrene Nanoparticles]]>Characterization of the physicochemical properties of Polystyrene NanoparticlesFabio Ugolinidx.doi.org/10.17504/protocols.io.m6gp8eqxdlzp/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.m6gp8eqxdlzp/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.m6gp8eqxdlzp/v1www.protocols.io/view/characterization-of-the-physicochemical-properties-kqyhcvxt7www.protocols.io/view/correction-to-high-throughput-hypocotyl-grafting-a-kqzecvx3fprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.1007/978-1-0716-5488-0_40

Correction to: High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in Arabidopsis Seedlings]]>Correction to: High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in Arabidopsis SeedlingsProtocols Importerdx.doi.org/10.1007/978-1-0716-5488-0_40protocols.io, Published online: 2026-10-28; | dx.doi.org/10.1007/978-1-0716-5488-0_402026-10-28protocols.iodx.doi.org/10.1007/978-1-0716-5488-0_40www.protocols.io/view/correction-to-high-throughput-hypocotyl-grafting-a-kqzecvx3fwww.protocols.io/view/determination-of-ic50-of-advanced-nanomaterials-ad-kqzicvx4fprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.54v98eow4l3e/v1

Determination of IC50 of Advanced nanomaterials (Ad-NMs) and nano-microplastics (NMPs) in vivo, in C. elegans]]>Determination of IC50 of Advanced nanomaterials (Ad-NMs) and nano-microplastics (NMPs) in vivo, in C. elegansFabio Ugolinidx.doi.org/10.17504/protocols.io.54v98eow4l3e/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.54v98eow4l3e/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.54v98eow4l3e/v1www.protocols.io/view/determination-of-ic50-of-advanced-nanomaterials-ad-kqzicvx4fwww.protocols.io/view/delphi-u23-24-week-physical-conditioning-schedule-kqzjcvx4pprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.q2lydzq1rlx9/v1

DELPHI U23 – 24-week physical conditioning schedule]]>DELPHI U23 – 24-week physical conditioning schedulePolina Rybakovadx.doi.org/10.17504/protocols.io.q2lydzq1rlx9/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.q2lydzq1rlx9/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.q2lydzq1rlx9/v1www.protocols.io/view/delphi-u23-24-week-physical-conditioning-schedule-kqzjcvx4pwww.protocols.io/view/protocol-for-determination-of-ic50-of-advanced-nan-kqztcvx6pprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.26g7rz968vwz/v1

Protocol for determination of IC50 of Advanced Nanomaterials and nano-microplastics in vitro using monocultures of cells]]>Protocol for determination of IC50 of Advanced Nanomaterials and nano-microplastics in vitro using monocultures of cellsFabio Ugolinidx.doi.org/10.17504/protocols.io.26g7rz968vwz/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.26g7rz968vwz/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.26g7rz968vwz/v1www.protocols.io/view/protocol-for-determination-of-ic50-of-advanced-nan-kqztcvx6pwww.protocols.io/view/determination-of-sub-acute-toxicity-of-advanced-na-kqzycvx7xprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.xmvm7omq9v3p/v1

Determination of sub-acute toxicity of advanced nanomaterials and nano-microplastics in vivo in C. elegans]]>Determination of sub-acute toxicity of advanced nanomaterials and nano-microplastics in vivo in C. elegansFabio Ugolinidx.doi.org/10.17504/protocols.io.xmvm7omq9v3p/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.xmvm7omq9v3p/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.xmvm7omq9v3p/v1www.protocols.io/view/determination-of-sub-acute-toxicity-of-advanced-na-kqzycvx7xwww.protocols.io/view/graphene-oxide-cellular-uptake-by-hrtem-kqz6cvx9fprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.6wgq9rqyylk5/v1

Graphene oxide cellular uptake by HRTEM]]>Graphene oxide cellular uptake by HRTEMFabio Ugolinidx.doi.org/10.17504/protocols.io.6wgq9rqyylk5/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.6wgq9rqyylk5/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.6wgq9rqyylk5/v1www.protocols.io/view/graphene-oxide-cellular-uptake-by-hrtem-kqz6cvx9fwww.protocols.io/view/determination-of-intracellular-localization-of-flu-kqz7cvx9pprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.r3l25mpbpl1y/v1

Determination of intracellular localization of fluorescent nanoparticles]]>Determination of intracellular localization of fluorescent nanoparticlesFabio Ugolinidx.doi.org/10.17504/protocols.io.r3l25mpbpl1y/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.r3l25mpbpl1y/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.r3l25mpbpl1y/v1www.protocols.io/view/determination-of-intracellular-localization-of-flu-kqz7cvx9pwww.protocols.io/view/ultra-long-dna-extraction-for-umi-tagged-rrna-oper-kq2dcvya7protocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.n2bvjkk8wgk5/v2

Ultra-long DNA Extraction for UMI-Tagged rRNA Operon Amplification Nanopore sequencing]]>Ultra-long DNA Extraction for UMI-Tagged rRNA Operon Amplification Nanopore sequencingKaiqin Biandx.doi.org/10.17504/protocols.io.n2bvjkk8wgk5/v2protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.n2bvjkk8wgk5/v22026-10-27protocols.iodx.doi.org/10.17504/protocols.io.n2bvjkk8wgk5/v2www.protocols.io/view/ultra-long-dna-extraction-for-umi-tagged-rrna-oper-kq2dcvya7www.protocols.io/view/detecci-n-molecular-de-la-variante-fq188r-c-563a-g-kq2ecvybfprotocols.io, Published online: 2026-10-27; doi:dx.doi.org/10.17504/protocols.io.m7vznmq24vx1/v1

Detección molecular de la variante FQ188R (c.563A>G), gen CFTR cromosoma 7 región 7q31; mediante PCR en tiempo real con tecnología TaqMan™ y verificación con Luna Universal Probe qPCR Master Mix]]>Detección molecular de la variante FQ188R (c.563A>G), gen CFTR cromosoma 7 región 7q31; mediante PCR en tiempo real con tecnología TaqMan™ y verificación con Luna Universal Probe qPCR Master MixDelia Piedad Recalde-Reyesdx.doi.org/10.17504/protocols.io.m7vznmq24vx1/v1protocols.io, Published online: 2026-10-27; | dx.doi.org/10.17504/protocols.io.m7vznmq24vx1/v12026-10-27protocols.iodx.doi.org/10.17504/protocols.io.m7vznmq24vx1/v1www.protocols.io/view/detecci-n-molecular-de-la-variante-fq188r-c-563a-g-kq2ecvybfwww.protocols.io/view/efficacy-and-life-expectancy-in-glioblastoma-patie-kq3ecvyjfprotocols.io, Published online: 2026-10-28; doi:dx.doi.org/10.17504/protocols.io.61ge6p8kdl47/v1

Efficacy and life expectancy in glioblastoma patients with oncolytic virus intervention: A systematic review of clinical cases.]]>Efficacy and life expectancy in glioblastoma patients with oncolytic virus intervention: A systematic review of clinical cases.Alexandre Duarte Pierantonidx.doi.org/10.17504/protocols.io.61ge6p8kdl47/v1protocols.io, Published online: 2026-10-28; | dx.doi.org/10.17504/protocols.io.61ge6p8kdl47/v12026-10-28protocols.iodx.doi.org/10.17504/protocols.io.61ge6p8kdl47/v1www.protocols.io/view/efficacy-and-life-expectancy-in-glioblastoma-patie-kq3ecvyjfwww.protocols.io/view/chromium-next-gem-single-cell-3-39-ht-reagent-kits-kq4tcvywpprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.4egnxbyozl5d/v1

Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) (GC000416 Rev D)-- University of Minnesota TMCs]]>Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) (GC000416 Rev D)-- University of Minnesota TMCsMac Andersondx.doi.org/10.17504/protocols.io.4egnxbyozl5d/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.4egnxbyozl5d/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.4egnxbyozl5d/v1www.protocols.io/view/chromium-next-gem-single-cell-3-39-ht-reagent-kits-kq4tcvywpwww.protocols.io/view/dna-extraction-with-zymobiomics-miniprep-kit-kq5ucvy6xprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.81wgbxjn1lpk/v2

DNA Extraction with ZymoBIOMICS MiniPrep Kit]]>DNA Extraction with ZymoBIOMICS MiniPrep KitCarlos Carlos Gollerdx.doi.org/10.17504/protocols.io.81wgbxjn1lpk/v2protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.81wgbxjn1lpk/v22026-10-29protocols.iodx.doi.org/10.17504/protocols.io.81wgbxjn1lpk/v2www.protocols.io/view/dna-extraction-with-zymobiomics-miniprep-kit-kq5ucvy6xwww.protocols.io/view/single-cell-96-well-tcr-seq-for-mouse-t-cells-kq52cvy8fprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.6wgq9rqzklk5/v1

Single cell 96-well TCR-seq for mouse T cells]]>Single cell 96-well TCR-seq for mouse T cellsRunze XIedx.doi.org/10.17504/protocols.io.6wgq9rqzklk5/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.6wgq9rqzklk5/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.6wgq9rqzklk5/v1www.protocols.io/view/single-cell-96-well-tcr-seq-for-mouse-t-cells-kq52cvy8fwww.protocols.io/view/pixel-seqv2-mouse-kidney-source-selection-tissue-p-kq6ccvzaxprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.p2l64ky51vqe/v1

Pixel-seqV2 mouse kidney: source selection, tissue preparation and assay]]>Pixel-seqV2 mouse kidney: source selection, tissue preparation and assayLi Sundx.doi.org/10.17504/protocols.io.p2l64ky51vqe/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.p2l64ky51vqe/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.p2l64ky51vqe/v1www.protocols.io/view/pixel-seqv2-mouse-kidney-source-selection-tissue-p-kq6ccvzaxwww.protocols.io/view/immunopixel-seqv2-mouse-kidney-source-selection-ti-kq6dcvza7protocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.8nlk5brqwl5r/v1

ImmunoPixel-seqV2 mouse kidney: source selection, tissue preparation and assay]]>ImmunoPixel-seqV2 mouse kidney: source selection, tissue preparation and assayLi Sundx.doi.org/10.17504/protocols.io.8nlk5brqwl5r/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.8nlk5brqwl5r/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.8nlk5brqwl5r/v1www.protocols.io/view/immunopixel-seqv2-mouse-kidney-source-selection-ti-kq6dcvza7www.protocols.io/view/development-and-scientific-rationale-of-a-caffeine-kq6tcvzepprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.2bvjrob4wlk5/v1

Development and Scientific Rationale of a Caffeine Supplementation Protocol to Enhance Performance in Freestyle Wrestlers with CYP1A2 Genetic Polymorphisms]]>Development and Scientific Rationale of a Caffeine Supplementation Protocol to Enhance Performance in Freestyle Wrestlers with CYP1A2 Genetic PolymorphismsPolina Rybakovadx.doi.org/10.17504/protocols.io.2bvjrob4wlk5/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.2bvjrob4wlk5/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.2bvjrob4wlk5/v1www.protocols.io/view/development-and-scientific-rationale-of-a-caffeine-kq6tcvzepwww.protocols.io/view/quantitative-dia-proteomic-analysis-kq7icvzkfprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.6nvw6pq37gmk/v1

Quantitative DIA Proteomic Analysis]]>Quantitative DIA Proteomic AnalysisMatthew Jaconellidx.doi.org/10.17504/protocols.io.6nvw6pq37gmk/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.6nvw6pq37gmk/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.6nvw6pq37gmk/v1www.protocols.io/view/quantitative-dia-proteomic-analysis-kq7icvzkfwww.protocols.io/view/yale-tmc-mouse-pan-organ-sample-selection-and-prep-kq7mcvzk7protocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.byl42e1oovo5/v1

Yale TMC - Mouse pan-organ sample selection and preparation protocol]]>Yale TMC - Mouse pan-organ sample selection and preparation protocolJungmin Namdx.doi.org/10.17504/protocols.io.byl42e1oovo5/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.byl42e1oovo5/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.byl42e1oovo5/v1www.protocols.io/view/yale-tmc-mouse-pan-organ-sample-selection-and-prep-kq7mcvzk7www.protocols.io/view/confirmation-of-in-vitro-p-rab-phosphorylation-sit-kq7ncvzmfprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.6wgq9rqwxlk5/v1

Confirmation of in vitro p-Rab Phosphorylation Sites by Mass Spectrometry]]>Confirmation of in vitro p-Rab Phosphorylation Sites by Mass SpectrometryMatthew Jaconellidx.doi.org/10.17504/protocols.io.6wgq9rqwxlk5/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.6wgq9rqwxlk5/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.6wgq9rqwxlk5/v1www.protocols.io/view/confirmation-of-in-vitro-p-rab-phosphorylation-sit-kq7ncvzmfwww.protocols.io/view/dry-frozen-foam-tongue-swab-sample-processing-and-kq7pcvzmpprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.r3l25mpwxl1y/v1

Dry frozen foam tongue swab sample processing and testing using the Pluslife MTB assay]]>Dry frozen foam tongue swab sample processing and testing using the Pluslife MTB assaySimon C Mendelsohndx.doi.org/10.17504/protocols.io.r3l25mpwxl1y/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.r3l25mpwxl1y/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.r3l25mpwxl1y/v1www.protocols.io/view/dry-frozen-foam-tongue-swab-sample-processing-and-kq7pcvzmpwww.protocols.io/view/methods-for-grc-scrnaseq-poster-wilmot-symposium-2-kq7rcvzm7protocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.wov1yjmryvr2/v1

Methods for GRC scRNAseq Poster - Wilmot Symposium 2026]]>Methods for GRC scRNAseq Poster - Wilmot Symposium 2026Hannah Aichelmandx.doi.org/10.17504/protocols.io.wov1yjmryvr2/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.wov1yjmryvr2/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.wov1yjmryvr2/v1www.protocols.io/view/methods-for-grc-scrnaseq-poster-wilmot-symposium-2-kq7rcvzm7www.protocols.io/view/single-nucleus-rna-sequencing-from-fresh-frozen-hu-kq8bcvzspprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.n92ld4oz7l5b/v2

Single-Nucleus RNA Sequencing from Fresh-Frozen Human Heart Tissue: Challenges and Optimization of Nuclei Isolation for the Chromium Fixed RNA Profiling (10x Genomics)]]>Single-Nucleus RNA Sequencing from Fresh-Frozen Human Heart Tissue: Challenges and Optimization of Nuclei Isolation for the Chromium Fixed RNA Profiling (10x Genomics)Lorena Rosasdx.doi.org/10.17504/protocols.io.n92ld4oz7l5b/v2protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.n92ld4oz7l5b/v22026-10-30protocols.iodx.doi.org/10.17504/protocols.io.n92ld4oz7l5b/v2www.protocols.io/view/single-nucleus-rna-sequencing-from-fresh-frozen-hu-kq8bcvzspwww.protocols.io/view/sop-antibody-validation-staining-transcription-fac-kq8vcvzw7protocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v1

SOP: ANTIBODY VALIDATION/STAINING Transcription Factor/Nuclear Staining]]>SOP: ANTIBODY VALIDATION/STAINING Transcription Factor/Nuclear StainingEduardo Mauriciodx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v1protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v12026-10-29protocols.iodx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v1www.protocols.io/view/sop-antibody-validation-staining-transcription-fac-kq8vcvzw7www.protocols.io/view/mass-cytometry-staining-of-single-cell-suspensions-kq8wcvzxfprotocols.io, Published online: 2026-10-29; doi:dx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v2

Mass Cytometry Staining of single cell suspensions]]>Mass Cytometry Staining of single cell suspensionsEduardo Mauriciodx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v2protocols.io, Published online: 2026-10-29; | dx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v22026-10-29protocols.iodx.doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v2www.protocols.io/view/mass-cytometry-staining-of-single-cell-suspensions-kq8wcvzxfwww.protocols.io/view/a-method-for-opening-stomata-and-measuring-photosy-kq8xcvzxpprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.92ld3bnong5b/v1

A Method For Opening Stomata And Measuring Photosynthetic Capacity]]>A Method For Opening Stomata And Measuring Photosynthetic CapacityRiley Leffdx.doi.org/10.17504/protocols.io.92ld3bnong5b/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.92ld3bnong5b/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.92ld3bnong5b/v1www.protocols.io/view/a-method-for-opening-stomata-and-measuring-photosy-kq8xcvzxpwww.protocols.io/view/nichedecode-workflow-for-inference-of-spatial-nich-kq86cvzzfprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v1

NicheDECODE workflow for inference of spatial niche abundances from tissue-level omics data]]>NicheDECODE workflow for inference of spatial niche abundances from tissue-level omics dataRenjie Liudx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v1www.protocols.io/view/nichedecode-workflow-for-inference-of-spatial-nich-kq86cvzzfwww.protocols.io/view/pre-flight-qc-for-fasta-genbank-and-fastq-files-be-kq87cvzzpprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.r3l25mpxxl1y/v1

Pre-flight QC for FASTA, GenBank, and FASTQ files before submission or analysis]]>Pre-flight QC for FASTA, GenBank, and FASTQ files before submission or analysisAndrew Floresdx.doi.org/10.17504/protocols.io.r3l25mpxxl1y/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.r3l25mpxxl1y/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.r3l25mpxxl1y/v1www.protocols.io/view/pre-flight-qc-for-fasta-genbank-and-fastq-files-be-kq87cvzzpwww.protocols.io/view/nichedecode-workflow-for-inference-of-spatial-nich-kq9scvz6fprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v2

NicheDECODE workflow for inference of spatial niche abundances from tissue-level omics data]]>NicheDECODE workflow for inference of spatial niche abundances from tissue-level omics dataRenjie Liudx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v2protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v22026-10-30protocols.iodx.doi.org/10.17504/protocols.io.6wgq9rq2xlk5/v2www.protocols.io/view/nichedecode-workflow-for-inference-of-spatial-nich-kq9scvz6fwww.protocols.io/view/a-novel-progressive-ratio-task-to-measure-effort-i-kraccv2axprotocols.io, Published online: 2026-10-30; doi:dx.doi.org/10.17504/protocols.io.jyl8nr8n6l2w/v1

A novel progressive ratio task to measure effort in early childhood and infancy]]>A novel progressive ratio task to measure effort in early childhood and infancyCaitlin Clementsdx.doi.org/10.17504/protocols.io.jyl8nr8n6l2w/v1protocols.io, Published online: 2026-10-30; | dx.doi.org/10.17504/protocols.io.jyl8nr8n6l2w/v12026-10-30protocols.iodx.doi.org/10.17504/protocols.io.jyl8nr8n6l2w/v1www.protocols.io/view/a-novel-progressive-ratio-task-to-measure-effort-i-kraccv2ax